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1. Fractional distillation under atmospheric pressure would most likely be used to separate which of the following compounds?
2. Which of the following compounds would be most effective in extracting benzoic acid from a diethyl ether solution?
3. What would be the effect on the Rf values if the thin-layer chromatography (TLC) described below were run with hexane rather than ether as the eluent?
4. If benzyl alcohol, benzyl acetate, p-nitrophenol, and naphthalene were separated by column chromatography with ether on silica gel, which compound would elute first according to the Rf values from question 3?
5. Which of the following would be the best procedure for extracting acetaldehyde from an aqueous solution?
6. Four compounds, I, II, III, and IV, are separated by chromatographic techniques. Compound III is the most polar, II the least polar, and I and IV have intermediate polarity. The solvent system is 85:15 ethanol:methylene chloride. Which spot on the card below likely belongs to compound III?
7. Suppose an extraction with methylene chloride is performed, with the desired compound initially in brine In a separatory funnel, which layer will be the organic layer?
8. Silica gel is often used in thin-layer chromatography. What property does silica gel probably possess that makes it useful for this purpose?
9. A mixture of sand, benzoic acid, and naphthalene in ether is best separated by:
10. Simple distillation could be used to separate which of the following compounds?
11. In order to separate a biological effector from solution, which chromatographic technique would be the most effective?
12. Given a solution of insulin (molecular weight = 5.8 kD) and titin (molecular weight = 3816 kD), which chromatographic technique would be the most effective for separating out usable molecules of titin?
13. The gas eluent in gas chromatography and the liquid eluent in paper chromatography are examples of which component of these systems?
14. During gravity filtration, a student forgets to heat the solution before running it through the filter. After capturing the filtrate, the student analyzes the sample via infrared (IR) spectroscopy and finds none of the desired product in the filtrate. What likely occurred to the student’s product?
15. Lactoferrin, a milk protein, is a valuable antimicrobial agent that is extracted from pasteurized, defatted milk utilizing a column containing a charged resin. This is an example of which of the following chromatographic techniques?
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