MCAT Biochemistry Question 8: Answer and Explanation
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Question: 8
8. Which of the following is least likely to cause denaturation of proteins?
- A. Heating the protein to 100°C
- B. Adding 8 M urea
- C. Moving it to a more hypotonic environment
- D. Adding a detergent such as sodium dodecyl sulfate
Correct Answer: C
Explanation:
High salt concentrations and detergents can denature a protein, as can high temperatures. But moving a protein to a hypotonic environment—that is, a lower solute concentration—should not lead to denaturation.