MCAT Organic Chemistry Question 176: Answer and Explanation
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Question: 176
11. In order to separate a biological effector from solution, which chromatographic technique would be the most effective?
- A. Thin-layer chromatography
- B. Ion-exchange chromatography
- C. Affinity chromatography
- D. Size-exclusion chromatography
Correct Answer: C
Explanation:
Affinity chromatography, using the target for the biological effector or a specific antibody, would work best in this case. It will specifically bind the protein of interest and keep it in the column.