MCAT Biochemistry Question 84: Answer and Explanation
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Question: 84
9. Restriction endonucleases are used for which of the following?
I. Gene therapy
II. Southern blotting
III. DNA repair
- A. I only
- B. II only
- C. II and III only
- D. I, II, and III
Correct Answer: D
Explanation:
Endonucleases are enzymes that cut DNA. They are used by the cell for DNA repair. They are also used by scientists during DNA analysis, as restriction enzymes are endonucleases. Restriction enzymes are used to cleave DNA before electrophoresis and Southern blotting, and to introduce a gene of interest into a viral vector for gene therapy.