MCAT Biochemistry Question 83: Answer and Explanation
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Question: 83
8. Which of the following statements regarding the polymerase chain reaction is FALSE?
- A. Human DNA polymerase is used because it is the most accurate.
- B. A primer must be prepared with a complementary sequence to part of the DNA of interest.
- C. Repeated heating and cooling cycles allow the enzymes to act specifically and replaces helicase.
- D. Each cycle of the polymerase chain reaction doubles the amount of DNA of interest.
Correct Answer: A
Explanation:
The polymerase chain reaction is used to clone a sequence of DNA using a DNA sample, a primer, free nucleotides, and enzymes. The polymerase from Thermus aquaticus is used because the reaction is regulated by thermal cycling, which would denature human enzymes.