MCAT Biochemistry Question 43: Answer and Explanation

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Question: 43

13. Which protein properties allow UV spectroscopy to be used as a method of determining concentration?

  • A. Proteins have partially planar characteristics in peptide bonds.
  • B. Globular proteins cause scattering of light.
  • C. Proteins contain aromatic groups in certain amino acids.
  • D. All organic macromolecules can be assessed with UV spectroscopy.

Correct Answer: C

Explanation:

UV spectroscopy is best used with conjugated systems of double bonds. While the double bond in the peptide bond does display resonance, this is not adequate for UV absorption. However, aromatic systems are conjugated, and phenylalanine, tyrosine, and tryptophan all contain aromatic ring structures.

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